ATP Citrate Lyase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15510

Novus Biologicals, part of Bio-Techne
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NBP2-15510
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATP Citrate Lyase Antibodies »

Product Summary for ATP Citrate Lyase Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ATP citrate lyase. The exact sequence is proprietary.

Localization

Cytoplasm;

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

121 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP Citrate Lyase Antibody

Western Blot: ATP Citrate Lyase Antibody [NBP2-15510]

Western Blot: ATP Citrate Lyase Antibody [NBP2-15510]

Western Blot: ATP Citrate Lyase Antibody [NBP2-15510] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP Citrate Lyase Antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody [NBP2-15510]

Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody [NBP2-15510]

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody [NBP2-15510] - ATP Citrate Lyase Antibody detects ATP Citrate Lyase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATP Citrate Lyase protein stained by ATP Citrate Lyase Antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP2-15510]

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP2-15510]

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP2-15510] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
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Applications for ATP Citrate Lyase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATP Citrate Lyase

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

ATP-citrate synthase

Alternate Names

ACLY, EC 2.3.3.8

Gene Symbol

ACLY

UniProt

P53396 (Human)

Additional ATP Citrate Lyase Products

Product Documents for ATP Citrate Lyase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP Citrate Lyase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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