Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ATP2C1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ATP2C1. The immunogen is located within the last 50 amino acids of ATP2C1. Amino Acid Squence: IKKVERSREKIQKHVS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%)

Specificity

At least four isoforms of ATP2C1 are known to exist; this antibody will recognize only the three longest isoforms. ATP2C1 antibody will not cross-react with ATP2C2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP2C1 Antibody - BSA Free

Western Blot: ATP2C1 AntibodyBSA Free [NBP1-76566]

Western Blot: ATP2C1 AntibodyBSA Free [NBP1-76566]

Western Blot: ATP2C1 Antibody [NBP1-76566] - mouse brain tissue lysate with ATP2C1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: ATP2C1 Antibody - BSA Free [NBP1-76566]

Immunocytochemistry/ Immunofluorescence: ATP2C1 Antibody - BSA Free [NBP1-76566]

Immunocytochemistry/Immunofluorescence: ATP2C1 Antibody [NBP1-76566] - Immunofluorescence of ATP2C1 in mouse brain tissue with ATP2C1 antibody at 20 ug/mL.
Immunohistochemistry: ATP2C1 Antibody - BSA Free [NBP1-76566]

Immunohistochemistry: ATP2C1 Antibody - BSA Free [NBP1-76566]

Immunohistochemistry: ATP2C1 Antibody [NBP1-76566] - Immunohistochemistry of ATP2C1 in mouse brain tissue with ATP2C1 antibody at 5 ug/mL.
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Applications for ATP2C1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP2C1

ATP2C1, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder characterized by persistent blisters and erosions of the skin. Unlike the related protein ATP2C2, ATP2C1 is ubiquitously expressed and displays a lower maximal turnover rate for overall Ca2+-ATPase reaction and a higher apparent affinity for cytosolic Ca2+ activation of phosphorylation. Recent evidence suggests that ATP2C1 is a key regulator of insulin-like growth factor receptor (IGF1R) processing in tumor progression in basal breast cancers.

Alternate Names

ATPase, Ca++ transporting, type 2C, member 1, BCPM, benign chronic pemphigus (Hailey-Hailey disease), Ca(2+)-sequestering, calcium-transporting ATPase type 2C member 1, EC 3.6.3, HHD, hSPCA1, HUSSY-28, PMR1L, secretory pathway Ca2+/Mn2+ ATPase 1

Gene Symbol

ATP2C1

UniProt

NP_001001486 (Human)

Additional ATP2C1 Products

Product Documents for ATP2C1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP2C1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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