ATP5F1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15513

Novus Biologicals, part of Bio-Techne
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NBP2-15513
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATP5F1 Antibodies »

Product Summary for ATP5F1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP5F1. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (89%), Bovine (83%).

Localization

Mitochondrion; Mitochondrion inner membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP5F1 Antibody

Western Blot: ATP5F1 Antibody [NBP2-15513]

Western Blot: ATP5F1 Antibody [NBP2-15513]

Western Blot: ATP5F1 Antibody [NBP2-15513] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: ATP5F1 Antibody [NBP2-15513]

Immunocytochemistry/ Immunofluorescence: ATP5F1 Antibody [NBP2-15513]

Immunocytochemistry/Immunofluorescence: ATP5F1 Antibody [NBP2-15513] - SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37 c for 30 min.Green: ATP synthase B1 protein stained by ATP synthase B1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: ATP5F1 Antibody [NBP2-15513]

Immunohistochemistry-Paraffin: ATP5F1 Antibody [NBP2-15513]

Immunohistochemistry-Paraffin: ATP5F1 Antibody [NBP2-15513] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Applications for ATP5F1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATP5F1

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq]

Alternate Names

ATP synthase B chain, mitochondrial, ATP synthase subunit b, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1, ATPase subunit b, cell proliferation-inducing protein 47, H+-ATP synthase subunit b, MGC24431, PIG47

Gene Symbol

ATP5PB

UniProt

P24539 (Human)

Additional ATP5F1 Products

Product Documents for ATP5F1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP5F1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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