ATP5I Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89496

Novus Biologicals, part of Bio-Techne
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NBP1-89496
NBP1-89496-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATP5I Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RRIAAEEKKKQDELKRIARELAEDDSILK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP5I Antibody

Western Blot: ATP5I Antibody [NBP1-89496]

Western Blot: ATP5I Antibody [NBP1-89496]

Western Blot: ATP5I Antibody [NBP1-89496] - Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: ATP5I Antibody [NBP1-89496]

Western Blot: ATP5I Antibody [NBP1-89496]

Western Blot: ATP5I Antibody [NBP1-89496] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATP5I antibody. Remaining relative intensity is presented
Immunocytochemistry/ Immunofluorescence: ATP5I Antibody [NBP1-89496]

Immunocytochemistry/ Immunofluorescence: ATP5I Antibody [NBP1-89496]

Immunocytochemistry/Immunofluorescence: ATP5I Antibody [NBP1-89496] - Staining of human cell line A-431 shows localization to mitochondria.Antibody staining is shown in green.
View 2 more images

Applications for ATP5I Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP5I

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the innermembrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalyticcore, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a singlerepresentative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodesthe e subunit of the F0 complex. (provided by RefSeq)

Alternate Names

ATP synthase e chain, mitochondrial, ATP synthase subunit e, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E, ATP5K, ATPase subunit e, F1F0-ATP synthase, murine e subunit, MGC12532

Gene Symbol

ATP5ME

Additional ATP5I Products

Product Documents for ATP5I Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP5I Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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