ATP5L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14332

Novus Biologicals, part of Bio-Techne
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NBP2-14332
NBP2-14332-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATP5L Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: AQFVRNLVEKTPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPRAIQSLKKIVNSAQTGSFK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP5L Antibody

Western Blot: ATP5L Antibody [NBP2-14332]

Western Blot: ATP5L Antibody [NBP2-14332]

Western Blot: ATP5L Antibody [NBP2-14332] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: ATP5L Antibody [NBP2-14332]

Immunocytochemistry/ Immunofluorescence: ATP5L Antibody [NBP2-14332]

Immunocytochemistry/Immunofluorescence: ATP5L Antibody [NBP2-14332] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: ATP5L Antibody [NBP2-14332]

Immunohistochemistry-Paraffin: ATP5L Antibody [NBP2-14332]

Immunohistochemistry-Paraffin: ATP5L Antibody [NBP2-14332] - Staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.

Applications for ATP5L Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP5L

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the F0 complex. (provided by RefSeq)

Alternate Names

ATP synthase subunit g, mitochondrial, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G, ATP5JG

Gene Symbol

ATP5MG

Additional ATP5L Products

Product Documents for ATP5L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP5L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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