ATP6AP1 Antibody (5G12A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27174

Novus Biologicals, part of Bio-Techne
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NBP3-27174-0.025ml
NBP3-27174-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G12A4

Concentration

1 mg/ml

View all ATP6AP1 Antibodies »

Product Summary for ATP6AP1 Antibody (5G12A4)

Immunogen

Purified recombinant fragment of human ATP6AP1 (AA: 51-151) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP6AP1 Antibody (5G12A4)

Western Blot: ATP6AP1 Antibody (5G12A4) [NBP3-27174] -

Western Blot: ATP6AP1 Antibody (5G12A4) [NBP3-27174] - Analysis using ATP6AP1 mAb against human ATP6AP1 (AA: 51-151) recombinant protein. (Expected MW is 37kDa)

ELISA: ATP6AP1 Antibody (5G12A4) [NBP3-27174] -

ELISA: ATP6AP1 Antibody (5G12A4) [NBP3-27174] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: ATP6AP1 Antibody (5G12A4) [NBP3-27174] -

Western Blot: ATP6AP1 Antibody (5G12A4) [NBP3-27174] - Analysis using ATP6AP1 mAb against HEK293-6e (1) and human ATP6AP1 (AA: 51-151)-hIgGFc transfected HEK293-6e (2) cell lysate.
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Applications for ATP6AP1 Antibody (5G12A4)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP6AP1

ATP6AP1 encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules.

Alternate Names

16A, Ac45, ATP6IP1V-ATPase subunit S1, ATP6S1ORF, ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1, ATPase, H+ transporting, lysosomal accessory protein 1, H+ transporting, lysosomal interacting protein 1, H-ATPase subunit, MGC129781, Protein XAP-3, Vacuolar proton pump subunit S1, VATPS1V-ATPase Ac45 subunit, V-type proton ATPase subunit S1, XAP-3, XAP3V-ATPase S1 accessory protein

Gene Symbol

ATP6AP1

Additional ATP6AP1 Products

Product Documents for ATP6AP1 Antibody (5G12A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP6AP1 Antibody (5G12A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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