ATP6V0D1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15517

Novus Biologicals, part of Bio-Techne
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NBP2-15517
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Key Product Details

Species Reactivity

Validated:

Human, Zebrafish

Predicted:

Bovine (100%), Chicken (98%), Mouse (99%), Rat (99%), Xenopus (95%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATP6V0D1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP6V0D1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (95%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP6V0D1 Antibody

Western Blot: ATP6V0D1 Antibody [NBP2-15517]

Western Blot: ATP6V0D1 Antibody [NBP2-15517]

Western Blot: ATP6V0D1 Antibody [NBP2-15517] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:500.
ATP6V0D1 Antibody

Immunohistochemistry: ATP6V0D1 Antibody [NBP2-15517] -

Immunohistochemistry: ATP6V0D1 Antibody [NBP2-15517] - Immunohistochemical analysis of agarose-embedded zebrafish embryo, using ATP6V0D1 antibody (NBP2-15517) at 1:200 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Applications for ATP6V0D1 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATP6V0D1

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [provided by RefSeq]

Alternate Names

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0, ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d isoform 1, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1, FLJ43534, H(+)-transporting two-sector ATPase, subunit D, P39, Vacuolar proton pump subunit d 1, V-ATPase 40 kDa accessory protein, V-ATPase AC39 subunit, V-ATPase, subunit D, VATX, Vma6, VPATPDV-ATPase subunit d 1, V-type proton ATPase subunit d 1

Gene Symbol

ATP6V0D1

UniProt

P61421 (Human)

Additional ATP6V0D1 Products

Product Documents for ATP6V0D1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP6V0D1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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