ATP6V1B2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88890

Novus Biologicals, part of Bio-Techne
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NBP1-88890
NBP1-88890-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATP6V1B2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MALRAMRGIVNGAAPELPVPTGGPAVGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP6V1B2 Antibody

Immunohistochemistry-Paraffin: ATP6V1B2 Antibody [NBP1-88890]

Immunohistochemistry-Paraffin: ATP6V1B2 Antibody [NBP1-88890]

Immunohistochemistry-Paraffin: ATP6V1B2 Antibody [NBP1-88890] - Staining in human cerebral cortex and skeletal muscle tissues using anti-ATP6V1B2 antibody. Corresponding ATP6V1B2 RNA-seq data are presented for the same tissues.
Western Blot: ATP6V1B2 Antibody [NBP1-88890]

Western Blot: ATP6V1B2 Antibody [NBP1-88890]

Western Blot: ATP6V1B2 Antibody [NBP1-88890] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-ATP6V1B2 antibody. Corresponding ATP6V1B2 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Immunocytochemistry/ Immunofluorescence: ATP6V1B2 Antibody [NBP1-88890]

Immunocytochemistry/ Immunofluorescence: ATP6V1B2 Antibody [NBP1-88890]

Immunocytochemistry/Immunofluorescence: ATP6V1B2 Antibody [NBP1-88890] - Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
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Applications for ATP6V1B2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP6V1B2

The ATP6V1B2 gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification ofeukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellularprocesses as protein sortin

Alternate Names

000 subunit, 56/58kD, isoform 2, ATP6B2ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide, ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2, Endomembrane proton pump 58 kDa subunit, H+ transporting two-sector ATPase, HO57ATP6B1B2, vacuolar H+-ATPase 56, Vacuolar proton pump subunit B 2, VATB, V-ATPase B2 subunit, V-ATPase subunit B 2, Vma2, VPP3ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2, V-type proton ATPase subunit B, brain isoform

Gene Symbol

ATP6V1B2

Additional ATP6V1B2 Products

Product Documents for ATP6V1B2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP6V1B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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