ATP6V1D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15518

Novus Biologicals, part of Bio-Techne
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NBP2-15518
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (98%), Chicken (93%), Rabbit (98%), Rat (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATP6V1D Antibodies »

Product Summary for ATP6V1D Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human V-ATPase D. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP6V1D Antibody

Western Blot: ATP6V1D Antibody [NBP2-15518]

Western Blot: ATP6V1D Antibody [NBP2-15518]

Western Blot: ATP6V1D Antibody [NBP2-15518] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-15518]

Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-15518]

Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-15518] - V-ATPase D antibody detects ATP6V1D protein at cytoplasm on Breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded Breast carcinoma. V-ATPase D antibody dilution: 1:500.
Western Blot: ATP6V1D Antibody [NBP2-15518]

Western Blot: ATP6V1D Antibody [NBP2-15518]

Western Blot: ATP6V1D Antibody [NBP2-15518] - V-ATPase D antibody detects ATP6V1D protein by Western blot analysis. A. 30 ug U87-MG whole cell lysate/extract 12 % SDS-PAGEV-ATPase D antibody dilution: 1:1000.

Applications for ATP6V1D Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATP6V1D

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq]

Alternate Names

ATP6M, ATPase, H+ transporting lysosomal, member M, ATPase, H+ transporting, lysosomal (vacuolar proton pump), ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D, H(+)-transporting two-sector ATPase, subunit M, vacuolar ATP synthase subunit D, vacuolar H-ATPase subunit D, vacuolar proton pump D subunit, vacuolar proton pump delta polypeptide, Vacuolar proton pump subunit D, vacuolar proton-ATPase subunit D, VATDV-ATPase D subunit, V-ATPase 28 kDa accessory protein, V-ATPase subunit D, VMA8, V-type proton ATPase subunit D

Gene Symbol

ATP6V1D

UniProt

Q9Y5K8 (Human)

Additional ATP6V1D Products

Product Documents for ATP6V1D Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ATP6V1D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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