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ATP6V1H Antibody (HL1316) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25372

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25372

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Drosophila

Predicted:

Bovine (98%), Chicken (96%), Porcine (99%), Xenopus (94%), Zebrafish (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1316

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for ATP6V1H Antibody (HL1316) - Azide and BSA Free

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP6V1H Antibody (HL1316) - Azide and BSA Free

ATP6V1H Antibody (HL1316) - Azide and BSA Free

Western Blot: ATP6V1H Antibody (HL1316) - Azide and BSA Free [NBP3-25372] -

Western Blot: ATP6V1H Antibody (HL1316) - Azide and BSA Free [NBP3-25372] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [HL1316] (NBP3-25372) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ATP6V1H Antibody (HL1316) - Azide and BSA Free

Immunohistochemistry-Paraffin: ATP6V1H Antibody (HL1316) - Azide and BSA Free [NBP3-25372] -

Immunohistochemistry-Paraffin: ATP6V1H Antibody (HL1316) - Azide and BSA Free [NBP3-25372] - ATP6V1H antibody [HL1316] detects ATP6V1H protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ATP6V1H stained by ATP6V1H antibody [HL1316] (NBP3-25372) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATP6V1H Antibody (HL1316) - Azide and BSA Free

Western Blot: ATP6V1H Antibody (HL1316) - Azide and BSA Free [NBP3-25372] -

Western Blot: ATP6V1H Antibody (HL1316) - Azide and BSA Free [NBP3-25372] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [HL1316] (NBP3-25372) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for ATP6V1H Antibody (HL1316) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP6V1H

ATP6V1H, also known as V-type proton ATPase subunit H, is a 483 amino acid protein that is a subunit of the V1 complex, which is responsible for acidifying eukaryotic cells in intracellular organelles. Studies on this protein have shown a relationship with the following disorders and diseases, osteopetrosis, carcinoma, cholera, colon carcinoma, arthritis, rheumatoid arthritis, neuronitis, tuberculosis, and immunodeficiency. The ATP6V1H protein has also shown an interaction with ATP6V1D, ATP6V1F, ATP6V1B2, ATP6V1E1, and ETHE1 in the oxidative phosphorylation, metabolic, phagosome, lysosome, insulin receptor recycline, and Iron uptake and transport pathways.

Alternate Names

ATPase, H+ transporting, lysosomal 50/57kD, V1 subunit H, ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H, CGI-11, MSTP042, NBP1, Nef-binding protein 1, Protein VMA13 homolog, SFD, SFDalpha, SFDbeta, vacuolar ATP synthase subunit H, vacuolar ATPase subunit H, vacuolar proton pump H subunit, Vacuolar proton pump subunit H, Vacuolar proton pump subunit SFD, V-ATPase 50/57 kDa subunits, V-ATPase H subunit, V-ATPase subunit H, VMA13, V-type proton ATPase subunit H

Gene Symbol

ATP6V1H

Additional ATP6V1H Products

Product Documents for ATP6V1H Antibody (HL1316) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATP6V1H Antibody (HL1316) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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