ATP7b Antibody (36D12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26488
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 36D12
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ATP7b Antibody (36D12)
Immunogen
A synthesized peptide derived from Human ATP7b [UniProt P35670]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ATP7b Antibody (36D12)
Immunohistochemistry: ATP7b Antibody (36D12) [NBP3-26488] -
Immunohistochemistry: ATP7b Antibody (36D12) [NBP3-26488] - Image of ATP7b Antibody (36D12) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.19% DAB.
Immunohistochemistry: ATP7b Antibody (36D12) [NBP3-26488] -
Immunohistochemistry: ATP7b Antibody (36D12) [NBP3-26488] - Image of ATP7b Antibody (36D12) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.19% DAB.
Immunocytochemistry/Immunofluorescence: ATP7b Antibody (36D12) [NBP3-26488] -
Immunocytochemistry/Immunofluorescence: ATP7b Antibody (36D12) [NBP3-26488] - Staining of HepG2 with ATP7b Antibody (36D12) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 494-conjugated Goat Anti-Rabbit IgG (H+L).Applications for ATP7b Antibody (36D12)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: ATP7b
Alternate Names
ATPase, Cu(2+)- transporting, beta polypeptide, ATPase, Cu++ transporting, beta polypeptide, Copper pump 2, copper-transporting ATPase 2, EC 3.6.3, EC 3.6.3.4, PWD, WC1, WD, Wilson disease-associated protein, WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)
Gene Symbol
ATP7B
Additional ATP7b Products
Product Documents for ATP7b Antibody (36D12)
Product Specific Notices for ATP7b Antibody (36D12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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