ATPase Na+/K+ beta 3 Antibody (M016)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23433

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M016

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATPase Na+/K+ beta 3 Antibodies »

Product Specifications

Immunogen

Clone (M016) was generated from a proprietary antigen related to the native human ATPase Na+/K+ beta 3 subunit expressed in A431 epidermoid carcinoma cell line. [UniProt# P54709]

Specificity

Clone M016 mouse monoclonal antibody detects a 40 kDa protein on SDS-PAGE "Native" immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured ATPase Na+/K+ beta 3 subunit. In addition, mass spectrometry analysis of immunoprecipitates using NM0161 in human A431 cell lysates confirms that this antibody only detects ATPase Na+/K+ beta 3 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for ATPase Na+/K+ beta 3 Antibody (M016)

ATPase Na+/K+ beta 3 Antibody (M016)

Immunocytochemistry/Immunofluorescence: ATPase Na+/K+ beta 3 Antibody (M016) [NBP3-23433] -

Immunocytochemistry/Immunofluorescence: ATPase Na+/K+ beta 3 Antibody (M016) [NBP3-23433] - Immunocytochemical labeling of ATPase Na+/K+ beta 3 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal anti-ATPase Na+/K+ beta 3 (clone M016). The antibody was detected using goat anti-mouse Ig DyLight(R) 594.
ATPase Na+/K+ beta 3 Antibody (M016)

Western Blot: ATPase Na+/K+ beta 3 Antibody (M016) [NBP3-23433] -

Western Blot: ATPase Na+/K+ beta 3 Antibody (M016) [NBP3-23433] - Western blot of NBP3-23433 immunoprecipitates (IP) and whole lysates. The NBP3-23433 antibody only (lane 1), IP from A431 cell lysate (lane 2), A431 cell input (lane 3), LNCaP cells (lane 4), MeWo cells (lane 5), and normal human lung (lane 6) and skin (lane 7). The blot was probed with anti-ATPase Na+/K+ beta 3 NBP3-23433 (lanes 1-7). The arrow designates native antibody, while the beta 3 subunit migrates around 40 kDa.

Applications for ATPase Na+/K+ beta 3 Antibody (M016)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunoprecipitation

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: ATPase Na+/K+ beta 3

ATPase Na+/ K+ beta 3 is encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2. [provided by RefSeq]

Long Name

Sodium/potassium-transporting ATPase subunit beta-3

Alternate Names

ATP1B3, ATPB-3, CD antigen CD298

Gene Symbol

ATP1B3

Additional ATPase Na+/K+ beta 3 Products

Product Documents for ATPase Na+/K+ beta 3 Antibody (M016)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATPase Na+/K+ beta 3 Antibody (M016)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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