ATPB Antibody (1H2W2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15354

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15354-100ul
NBP3-15354-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1H2W2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATPB Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATPB (NP_001677.2). YSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATPB Antibody (1H2W2)

Immunohistochemistry-Paraffin: ATPB Antibody (1H2W2) [NBP3-15354]

Immunohistochemistry-Paraffin: ATPB Antibody (1H2W2) [NBP3-15354]

Immunohistochemistry-Paraffin: ATPB Antibody (9A1O2) [NBP3-15354] - Immunohistochemistry of paraffin-embedded human esophageal cancer using ATPB Rabbit mAb (NBP3-15354) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ATPB Antibody (1H2W2) [NBP3-15354]

Immunohistochemistry-Paraffin: ATPB Antibody (1H2W2) [NBP3-15354]

Immunohistochemistry-Paraffin: ATPB Antibody (9A1O2) [NBP3-15354] - Immunohistochemistry of paraffin-embedded rat kidney using ATPB Rabbit mAb (NBP3-15354) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ATPB Antibody (9A1O2)

Western Blot: ATPB Antibody (9A1O2) [NBP3-15354] -

Western Blot: ATPB Antibody (9A1O2) [NBP3-15354] - analysis of extracts of various cell lines, using ATPB antibody at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
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Applications for ATPB Antibody (1H2W2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:3000 - 1:20000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATPB

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

Alternate Names

ATP synthase subunit beta, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, ATPMB, ATPSBmitochondrial ATP synthase beta subunit, EC 3.6.3, EC 3.6.3.14, MGC5231, mitochondrial ATP synthetase, beta subunit

Gene Symbol

ATP5F1B

Additional ATPB Products

Product Documents for ATPB Antibody (1H2W2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATPB Antibody (1H2W2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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