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ATR Antibody (4O3E0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33305

Novus Biologicals, part of Bio-Techne
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NBP3-33305-100ul
NBP3-33305-20ul

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 4O3E0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 2100-2200 of human ATR (NP_001175.2).

Sequence:
KAYEWEKAGRSDRVQMRNDLGKINKVITEHTNYLAPYQFLTAFSQLISRICHSHDEVFVVLMEIIAKVFLAYPQQAMWMMTAVSKSSYPMRVNRCKEILNK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

301 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATR Antibody (4O3E0)

ATR Antibody (4O3E0)

Western Blot: ATR Antibody (4O3E0) [NBP3-33305] -

Western Blot: ATR Antibody (4O3E0) [NBP3-33305] - Western blot analysis of various lysates using ATR Rabbit mAb at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Applications for ATR Antibody (4O3E0)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATR

Ataxia Telangiectasia Mutated (ATM) and Rad3- related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa, and it has been shown to be involved in cellular responses to DNA damage. Specifically, it plays a role in checkpoint activation in response to either the presence of stalled replication forks, or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice.

Long Name

Ataxia Telangiectasia and Rad 3-related

Alternate Names

FRP1, MEC1, SCKL1

Gene Symbol

ATR

Additional ATR Products

Product Documents for ATR Antibody (4O3E0)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATR Antibody (4O3E0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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