Atrial Natriuretic Peptide/ANP Antibody (2C5S5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33325
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 2C5S5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Atrial Natriuretic Peptide/ANP (NP_006163.1).
Sequence:
MSSFSTTTVSFLLLLAFQLLGQTRANPMYNAVSNADLMDFKNLLDHLEEKMPLEDEVVPPQVLSEPNEEAGAALSPLPEVPPWTGEVSPAQRDGGALGRG
Sequence:
MSSFSTTTVSFLLLLAFQLLGQTRANPMYNAVSNADLMDFKNLLDHLEEKMPLEDEVVPPQVLSEPNEEAGAALSPLPEVPPWTGEVSPAQRDGGALGRG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Atrial Natriuretic Peptide/ANP Antibody (2C5S5)
Western Blot: Atrial Natriuretic Peptide/ANP Antibody (2C5S5) [NBP3-33325] -
Western Blot: Atrial Natriuretic Peptide/ANP Antibody (2C5S5) [NBP3-33325] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with NPPA using NPPA Rabbit mAb at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry: Atrial Natriuretic Peptide/ANP Antibody (2C5S5) [NBP3-33325] -
Immunohistochemistry: Atrial Natriuretic Peptide/ANP Antibody (2C5S5) [NBP3-33325] - Confocal imaging of paraffin-embedded mouse heart using NPPA Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(Red). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.Applications for Atrial Natriuretic Peptide/ANP Antibody (2C5S5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:2000 - 1:6000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Atrial Natriuretic Peptide/ANP
Alternate Names
ANF, ANP, Cardionatrin, NPPA, PND
Gene Symbol
NPPA
Additional Atrial Natriuretic Peptide/ANP Products
Product Documents for Atrial Natriuretic Peptide/ANP Antibody (2C5S5)
Product Specific Notices for Atrial Natriuretic Peptide/ANP Antibody (2C5S5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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