Atrial Natriuretic Peptide/ANP Antibody (GT1068)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13605

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT1068

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Atrial Natriuretic Peptide/ANP Antibodies »

Product Specifications

Immunogen

Full length human Atrial Natriuretic Peptide/ANP recombinant protein.

Reactivity Notes

Rat (80%), Dog (85%), Pig (87%), Rabbit (81%), Bovine (85%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Atrial Natriuretic Peptide/ANP Antibody (GT1068)

Immunocytochemistry/ Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605]

Immunocytochemistry/ Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605]

Immunocytochemistry/Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ANP stained by ANP antibody [1068] iluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.
Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605]

Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605]

Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - Mouse heart. ANP stained by ANP antibody [1068] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Atrial Natriuretic Peptide/ANP Antibody (GT1068)

Western Blot: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] -

Western Blot: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Atrial Natriuretic Peptide/ANP antibody [GT1068] (NBP3-13605) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for Atrial Natriuretic Peptide/ANP Antibody (GT1068)

Application
Recommended Usage

Western Blot

1:500-1:3000
Application Notes
IHC, ICC/IF-Assay dependent

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS pH7, 20% Glycerol

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Atrial Natriuretic Peptide/ANP

Atrial natriuretic factor (ANP) is a potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis and has a cGMP stimulating activity. There are two different prepronatriodilatin alleles. One codes for 2 Arginine residues at the C terminus that are cleaved to form the mature peptide, while the other ends in a termination codon immediately after the last codon of the mature peptide. ANP belongs to the natriuretic peptide family.

Alternate Names

ANF, ANP, Cardionatrin, NPPA, PND

Gene Symbol

NPPA

Additional Atrial Natriuretic Peptide/ANP Products

Product Documents for Atrial Natriuretic Peptide/ANP Antibody (GT1068)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Atrial Natriuretic Peptide/ANP Antibody (GT1068)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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