ATRX Antibody (rATRX/3446)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07643

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rATRX/3446

Concentration

0.2 mg/ml

View all ATRX Antibodies »

Product Specifications

Immunogen

Recombinant full-length human ATRX protein (Uniprot: P46100 )

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

280 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08673)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ATRX Antibody (rATRX/3446)

Immunohistochemistry-Paraffin: ATRX Antibody (rATRX/3446) [NBP3-07643]

Immunohistochemistry-Paraffin: ATRX Antibody (rATRX/3446) [NBP3-07643]

Immunohistochemistry-Paraffin: ATRX Antibody (rATRX/3446) [NBP3-07643] - Formalin-fixed, paraffin-embedded human glioma stained with ATRX Antibody (rATRX/3446).
Immunohistochemistry-Paraffin: ATRX Antibody (rATRX/3446) [NBP3-07643]

Immunohistochemistry-Paraffin: ATRX Antibody (rATRX/3446) [NBP3-07643]

Immunohistochemistry-Paraffin: ATRX Antibody (rATRX/3446) [NBP3-07643] - Formalin-fixed, paraffin-embedded human prostate stained with ATRX Recombinant Mouse Monoclonal Antibody (rATRX/3446).
ATRX Antibody (rATRX/3446)

ATRX Antibody (rATRX/3446)

Formalin-fixed, paraffin-embedded human prostate stained with ATRX antibody (rATRX/3446).

Applications for ATRX Antibody (rATRX/3446)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ATRX

ATRX is encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.

Alternate Names

alpha thalassemia/mental retardation syndrome X-linked, alpha thalassemia/mental retardation syndrome X-linked (RAD54 (S. cerevisiae)homolog), alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S.cerevisiae), ATP-dependent helicase ATRX, ATR2, DNA dependent ATPase and helicase, EC 3.6.1, EC 3.6.4.12, helicase 2, X-linked, Juberg-Marsidi syndrome, MGC2094, MRXHF1, RAD54 homolog, RAD54L, SFM1, SHS, transcriptional regulator ATRX, XH2RAD54, X-linked helicase II, X-linked nuclear protein, XNPZNF-HX, Zinc finger helicase, Znf-HX

Gene Symbol

ATRX

UniProt

P46100 (Human)

Additional ATRX Products

Product Documents for ATRX Antibody (rATRX/3446)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATRX Antibody (rATRX/3446)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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