AUF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15528

Novus Biologicals, part of Bio-Techne
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NBP2-15528
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (98%), Rat (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AUF1 Antibodies »

Product Summary for AUF1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human AUF1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (82%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AUF1 Antibody

Western Blot: AUF1 Antibody [NBP2-15528]

Western Blot: AUF1 Antibody [NBP2-15528]

Western Blot: AUF1 Antibody [NBP2-15528] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: AUF1 Antibody [NBP2-15528]

Immunocytochemistry/ Immunofluorescence: AUF1 Antibody [NBP2-15528]

Immunocytochemistry/Immunofluorescence: AUF1 Antibody [NBP2-15528] - Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AUF1 protein stained by AUF1 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunocytochemistry/ Immunofluorescence: AUF1 Antibody [NBP2-15528]

Immunocytochemistry/ Immunofluorescence: AUF1 Antibody [NBP2-15528]

Immunocytochemistry/Immunofluorescence: AUF1 Antibody [NBP2-15528] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AUF1 protein stained by AUF1 antibody [N1C1] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.

Applications for AUF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AUF1

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq]

Alternate Names

AUF1A, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein1, 37kDa), heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA-binding protein1, 37kD), heterogeneous nuclear ribonucleoprotein D0, hnRNPD0, type A

Gene Symbol

HNRNPD

UniProt

Q14103 (Human)

Additional AUF1 Products

Product Documents for AUF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AUF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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