Aurora A Antibody (1A11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50040
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1A11
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Summary for Aurora A Antibody (1A11)
Immunogen
Full length recombinant human protein aurora A expressed in and purified from E. coli
Specificity
This antibody does not cross-react with Aurora B or Aurora C.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aurora A Antibody (1A11)
![Western Blot: Aurora A Antibody (1A11) [NBP2-50040]](https://resources.bio-techne.com/images/products/Aurora-A-Antibody-1A11-Western-Blot-NBP2-50040-img0005.jpg "Western Blot: Aurora A Antibody (1A11) [NBP2-50040]")
Western Blot: Aurora A Antibody (1A11) [NBP2-50040]
Western Blot: Aurora A Antibody (1A11) [NBP2-50040] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A mAb. Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6hrs: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The NBP2-50040 antibody reacts with the aurora A recombinant protein but not aurora B or C.![Immunocytochemistry/ Immunofluorescence: Aurora A Antibody (1A11) [NBP2-50040]](https://resources.bio-techne.com/images/products/Aurora-A-Antibody-1A11-Immunocytochemistry-Immunofluorescence-NBP2-50040-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Aurora A Antibody (1A11) [NBP2-50040]")
Immunocytochemistry/ Immunofluorescence: Aurora A Antibody (1A11) [NBP2-50040]
Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1A11) [NBP2-50040] - HeLa cell cultures stained with NBP2-50040 (green). Aurora A localizes in spindle poles and mitotic spindles at late mitosis. Counterstained is chicken polyclonal antibody against Vimentin (NB300-223, red). Blue is a DNA stain.Applications for Aurora A Antibody (1A11)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunohistochemistry
1:100-1:500
Western Blot
1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Supplied as concentrated hybridoma cell culture media.
Preservative
0.035% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aurora A
Additional Aurora A Products
Product Specific Notices for Aurora A Antibody (1A11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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