Aurora A/B Antibody (5A12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50049

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Canine, Equine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5A12

Concentration

1 mg/ml

View all Aurora A/B Antibodies »

Product Specifications

Immunogen

Full length recombinant human Aurora A protein expressed in and purified from E. coli. [UniProt# O14965]

Specificity

This antibody does not cross-react with Aurora C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46, 38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aurora A/B Antibody (5A12)

Western Blot: Aurora A/B Antibody (5A12) [NBP2-50049]

Western Blot: Aurora A/B Antibody (5A12) [NBP2-50049]

Western Blot: Aurora A/B Antibody (5A12) [NBP2-50049] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A/B mAb. Left: cells were treated with 100 ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: western blot of purified full length human recombinant aurora A, B, C preparations as indicated. Bands at 46kDa and 38kDa correspond to aurora A and B proteins but not C. NBP2-50049 reacts with aurora A an B but not C.
Immunocytochemistry/ Immunofluorescence: Aurora A/B Antibody (5A12) [NBP2-50049]

Immunocytochemistry/ Immunofluorescence: Aurora A/B Antibody (5A12) [NBP2-50049]

Immunocytochemistry/Immunofluorescence: Aurora A/B Antibody (5A12) [NBP2-50049] - HeLa cell cultures were stained with NBP2-50049 (green). The antibody stains spindle poles and mitotic spindles at anaphase (top 2 panels) and concentrates on the midbody between the two daughter cells during telophase (bottom 2 panels). It is therefore a useful marker of dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.

Applications for Aurora A/B Antibody (5A12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:2000

Immunohistochemistry

1:1000 - 1:2000

Western Blot

1:1000 - 1:2000
Application Notes
In WB, this antibody binds strongly to Aurora A at about 46 kDa and weakly to Aurora B at 38 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora A/B

Alternate Names

AIK;AIK2;AIM1;AIM-1;AIRK1;AIRK2;ARK1;ARK2;AURA;AurB;AURKA;AURKB;aurkb-sv1;aurkb-sv2;AYK1;BTAK;IAK1;IPL1;PPP1R47;PPP1R48;STK1;STK12;STK15;STK5;STK6;STK7

UniProt

O14965, Q96GD4

Additional Aurora A/B Products

Product Documents for Aurora A/B Antibody (5A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Aurora A/B Antibody (5A12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.