Autophagy Antibody Pack

Novus Biologicals, part of Bio-Techne | Catalog # NB910-94877

Novus Biologicals, part of Bio-Techne
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NB910-94877
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Key Product Details

Species

Human, Mouse, Rat, Porcine, Alligator, Bovine, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockdown Validated, Simple Western, Western Blot

Product Summary for Autophagy Antibody Pack

This pack contains 1 vial each of: NB100-2220 (25 uL), NB110-87318 (25 uL), NB110-53818 (25 uL), NB110-56893 (25 uL) and NB110-82384 (25 uL).

Product Specifications

Specificity

Autophagy Antibodies (Beclin, LC3, ATG5, ATG9, ATG16L1)

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for Autophagy Antibody Pack

Knockout Validated: Autophagy Antibody Pack [NB910-94877]

Knockout Validated: Autophagy Antibody Pack [NB910-94877]

Knockout Validated: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.
Knockout Validated: Autophagy Antibody Pack [NB910-94877]

Knockout Validated: Autophagy Antibody Pack [NB910-94877]

Knockout Validated: Autophagy Antibody Pack [NB910-94877] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Knockout Validated: Autophagy Antibody Pack [NB910-94877]

Knockout Validated: Autophagy Antibody Pack [NB910-94877]

Knockout Validated: Autophagy Antibody Pack [NB910-94877] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG antibody (NB110-53818) used.
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Formulation, Preparation, and Storage

Formulation

See individual datasheets

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Autophagy

This Autophagy Antibodies Pack includes sample aliquots to many different autophagy related proteins.
Autophagy can be understood as a cell recycling system on its most basic level. Components of the cytoplasm are sequestered and moved in the lysosome where they are broken down into their basic components and returned to the cytosol for reuse.

Alternate Names

ATG6, ATG6 autophagy related 6 homolog, beclin 1 (coiled-coil, moesin-like BCL2 interacting protein), beclin 1 (coiled-coil, moesin-like BCL2-interacting protein), beclin 1, autophagy related, beclin1, beclin-1, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197, VPS30

Gene Symbol

BECN1

Additional Autophagy Products

Product Documents for Autophagy Antibody Pack

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Autophagy Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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