Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3G4

Format

BSA Free

Concentration

1.0 mg/ml

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Product Specifications

Immunogen

Recombinant human AVEN (254-362aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for AVEN Antibody (3G4) - BSA Free

Western Blot: AVEN Antibody (3G4)BSA Free [NBP1-07098]

Western Blot: AVEN Antibody (3G4)BSA Free [NBP1-07098]

Western Blot: AVEN Antibody (3G4) [NBP1-07098] - The extracts of mouse kidney (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunohistochemistry-Paraffin: AVEN Antibody (3G4) - BSA Free [NBP1-07098]

Immunohistochemistry-Paraffin: AVEN Antibody (3G4) - BSA Free [NBP1-07098]

Immunohistochemistry-Paraffin: AVEN Antibody (3G4) [NBP1-07098] - Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Applications for AVEN Antibody (3G4) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AVEN

PDCD12 (Programmed cell death 12), also referred to as AVEN, represents a new class of cell death regulator. PDCD12 is a conserved intracellular membrane protein that blocks apoptosis impeded by Apaf-1 mediated caspase activation. Bcl-2 and BAD also interact with PDCD12 to prevent apoptosis. It is expressed in a wide variety of normal tissues including heart, skeletal muscle, kidney, liver, testis, and diseases such as cancer. Clone 3G4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDCD12 protein.

Alternate Names

apoptosis, caspase activation inhibitor, cell death regulator Aven, PDCD12, programmed cell death 12

Entrez Gene IDs

57099 (Human)

Gene Symbol

AVEN

UniProt

NP_065104 (Human)

Additional AVEN Products

Product Documents for AVEN Antibody (3G4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AVEN Antibody (3G4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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