AZ1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-68225

Novus Biologicals, part of Bio-Techne
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NB100-68225
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all AZ1 Antibodies »

Product Summary for AZ1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human centrosomal protein 131 kDa (5-azacytidine induced 1) using the numbering given in entry EAW89632.1 (GeneID 22994).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AZ1 Antibody

Western Blot: AZ1 Antibody [NB100-68225]

Western Blot: AZ1 Antibody [NB100-68225]

Western Blot: AZ1 Antibody [NB100-68225] - Detection of Human CEP131/AZ1 on HeLa whole cell lysate using NB100-68225. CEP131/AZ1 was also immunoprecipitated by rabbit anti-CEP131/AZ1 antibodies NB100-68226 and NB100-68227.
Immunocytochemistry/ Immunofluorescence: AZ1 Antibody [NB100-68225]

Immunocytochemistry/ Immunofluorescence: AZ1 Antibody [NB100-68225]

Immunocytochemistry/Immunofluorescence: AZ1 Antibody [NB100-68225] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:200.

Applications for AZ1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Immunofluorescence (ICC)- Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AZ1

CEP131/AZ1 was originally identified as a transcript that was increased in cells exposed to the demethylating agent, azacytidine. It was found to localize to the pre-acrosome region of spermatids and therefore thought to play a role in spermatogenesis. CEP131/AZ1 has also been identified as a component of the centrosome.

Alternate Names

5-azacytidine induced 1, 5-azacytidine-induced protein 1, AZ1, centrosomal protein 131 kDa, centrosomal protein of 131 kDa, Cep131, KIAA1118, pre-acrosome localization protein 1, ZA1

Entrez Gene IDs

22994 (Human)

Gene Symbol

CEP131

UniProt

EAW89632.1 (Human)

Additional AZ1 Products

Product Documents for AZ1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AZ1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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