B220/CD45R Antibody (F10-89-4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54455

Novus Biologicals, part of Bio-Techne
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NBP2-54455-100ug
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # F10-89-4

Format

Azide and BSA Free

Concentration

1 mg/ml

View all B220/CD45R Antibodies »

Product Specifications

Immunogen

Human T-Lymphocytes (Uniprot: P08575)

Localization

Cell surface and Cytoplasmic

Specificity

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53175).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for B220/CD45R Antibody (F10-89-4) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]

Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]

Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45 with B220/CD45R Antibody (F10-89-4) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]

Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]

Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455] - Formalin-fixed, paraffin-embedded human Tonsil stained with B220/CD45R Antibody (F10-89-4).
Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]

Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]

Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455] - Human Tonsil stained with CD45 Monoclonal Antibody (F10-89-4).
View 1 more images

Applications for B220/CD45R Antibody (F10-89-4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5 - 1 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: B220/CD45R

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Long Name

Cluster of Differentiation 45

Alternate Names

CD45R

Gene Symbol

PTPRC

Additional B220/CD45R Products

Product Documents for B220/CD45R Antibody (F10-89-4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B220/CD45R Antibody (F10-89-4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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