B220/CD45R Antibody (F10-89-4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54455
![Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-F10-89-4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54455-img0002.jpg "Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # F10-89-4
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Human T-Lymphocytes (Uniprot: P08575)
Localization
Cell surface and Cytoplasmic
Specificity
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53175).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for B220/CD45R Antibody (F10-89-4) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]
Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45 with B220/CD45R Antibody (F10-89-4) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.![Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-F10-89-4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54455-img0003.jpg "Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]")
Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]
Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455] - Formalin-fixed, paraffin-embedded human Tonsil stained with B220/CD45R Antibody (F10-89-4).![Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-F10-89-4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54455-img0001.jpg "Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]")
Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455]
Immunohistochemistry-Paraffin: B220/CD45R Antibody (F10-89-4) - Azide and BSA Free [NBP2-54455] - Human Tonsil stained with CD45 Monoclonal Antibody (F10-89-4).Applications for B220/CD45R Antibody (F10-89-4) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: B220/CD45R
Long Name
Cluster of Differentiation 45
Alternate Names
CD45R
Gene Symbol
PTPRC
Additional B220/CD45R Products
Product Documents for B220/CD45R Antibody (F10-89-4) - Azide and BSA Free
Product Specific Notices for B220/CD45R Antibody (F10-89-4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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