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B7-H2/ICOSLG Antibody (prezalumab) - IgG2SA

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28507

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG2 Clone # prezalumab

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

B7-H2 / ICOSL / CD275

Clonality

Monoclonal

Host

Human

Isotype

IgG2

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG2, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Also known as 'prezalumab'.

IgG2SA is human IgG2 with S378A mutation.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for B7-H2/ICOSLG Antibody (prezalumab) - IgG2SA

B7-H2/ICOSLG Antibody (prezalumab) - IgG2SA

ELISA: B7-H2/ICOSLG Antibody (prezalumab) - IgG2SA[NBP3-28507] -

Immobilized human B7 H2 His at 2 ug/mL can bind B7-H2/ICOSLG Antibody (prezalumab) - IgG2SA, EC50=0.007111 ug/mL.

Applications for B7-H2/ICOSLG Antibody (prezalumab) - IgG2SA

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: B7-H2

B7-RP1 is a 40 kD protein, also known as ICOS ligand, B7-H2, and B7h. It has recently been clustered as CD275. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells and macrophages. The expression of this antigen is increased by TNF- alpha stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.

Long Name

B7 Homolog 2

Alternate Names

B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG

Gene Symbol

ICOSLG

UniProt

Additional B7-H2 Products

Product Documents for B7-H2/ICOSLG Antibody (prezalumab) - IgG2SA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for B7-H2/ICOSLG Antibody (prezalumab) - IgG2SA

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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