B7-H3/CD276 Antibody (BLR025F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14669

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14669
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR025F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all B7-H3 Antibodies »

Product Summary for B7-H3/CD276 Antibody (BLR025F)

Immunogen

Immunogen was a peptide representing a region between residue 500 and the C-terminus (residue 534) of human CD276 Molecule using the numbering given in entry NP_001019907.1 (GeneID 80381).

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for B7-H3/CD276 Antibody (BLR025F)

Western Blot: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669]

Western Blot: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669]

Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (50 ug) from TCMK-1, NIH 3T3,AlphaTC1 Clone 9, 3T3-L1, and CT26 cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 minutes.
Immunocytochemistry/ Immunofluorescence: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669]

Immunocytochemistry/ Immunofluorescence: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669]

Immunocytochemistry/Immunofluorescence: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Section of A-172 cells. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.
Immunohistochemistry: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669]

Immunohistochemistry: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669]

Immunohistochemistry: B7-H3/CD276 Antibody [NBP3-14669] - Sample: FFPE section of humanlung carcinoma. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain:DAPI (blue).
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Applications for B7-H3/CD276 Antibody (BLR025F)

Application
Recommended Usage

Flow Cytometry

1 ul per 1 x 10^6 cells.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

3 ul/mg lysate

Western Blot

1:1000
Application Notes
1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with , BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: B7-H3

Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).

Long Name

B7 Homolog 3

Alternate Names

B7H3, CD276

Gene Symbol

CD276

Additional B7-H3 Products

Product Documents for B7-H3/CD276 Antibody (BLR025F)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B7-H3/CD276 Antibody (BLR025F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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