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BAAT Antibody (5B6)

Novus Biologicals, part of Bio-Techne | Catalog # H00000570-M02

Novus Biologicals, part of Bio-Techne
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H00000570-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

BAAT (NP_001692, 258 a.a. ~ 355 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NGTNFPFGIPQVYHGQIHQPLPHSAQLISTNALGLLELYRTFETTQVGASQYLFPIEEAQGQFLFIVGEGDKTINSKAHAEQAIGQLKRHGKNNWTLL

Specificity

BAAT - bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for BAAT Antibody (5B6)

Western Blot: BAAT Antibody (5B6) [H00000570-M02]

Western Blot: BAAT Antibody (5B6) [H00000570-M02]

Western Blot: BAAT Antibody (5B6) [H00000570-M02] - BAAT monoclonal antibody (M02), clone 5B6 Analysis of BAAT expression in HepG2.
Sandwich ELISA: BAAT Antibody (5B6) [H00000570-M02] - Detection limit for recombinant GST tagged BAAT is approximately 0.3ng/ml as a capture antibody.

Applications for BAAT Antibody (5B6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BAAT

The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene.

Alternate Names

BACAT, BATbile acid-CoA:amino acid N-acyltransferase, bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase), bile acid Coenzyme A: amino acid N-acyltransferase (glycineN-choloyltransferase), EC 2.3.1.65, EC 3.1.2.2, FLJ20300, Glycine N-choloyltransferase, Long-chain fatty-acyl-CoA hydrolase, MGC104432

Entrez Gene IDs

570 (Human)

Gene Symbol

BAAT

OMIM

602938 (Human)

Additional BAAT Products

Product Documents for BAAT Antibody (5B6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BAAT Antibody (5B6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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