BAF180/PB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32025

Novus Biologicals, part of Bio-Techne
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NBP1-32025
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BAF180/PB1 Antibodies »

Product Summary for BAF180/PB1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human BAF180/PB1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (81%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

193 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BAF180/PB1 Antibody

Western Blot: BAF180/PB1 Antibody [NBP1-32025]

Western Blot: BAF180/PB1 Antibody [NBP1-32025]

Western Blot: BAF180/PB1 Antibody [NBP1-32025] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE PB1 antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: BAF180/PB1 Antibody [NBP1-32025]

Immunocytochemistry/ Immunofluorescence: BAF180/PB1 Antibody [NBP1-32025]

Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody [NBP1-32025] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NBP1-32025]

Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NBP1-32025]

Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NBP1-32025] - Paraffin-embedded mouse duodenum. PB1 antibody [N1N2], N-term diluted at 1:500.

Applications for BAF180/PB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BAF180/PB1

The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PB1/BAF180 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PB1/BAF180 has been proposed to function as a tumor suppressor protein. Recently, PB1/BAF180 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21. Alternate names for PB1/BAF180 include protein polybromo-1, polybromo-1D, BRG1-associated factor 180, hPB1, and PBRM1.

Long Name

Protein polybromo-1

Alternate Names

BAF180, hPB1, PB1, PBRM1, Polybromo-1D

Gene Symbol

PBRM1

Additional BAF180/PB1 Products

Product Documents for BAF180/PB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BAF180/PB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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