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BAF53A Antibody (S03-1G4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19983

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19983-100ul
NBP3-19983-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S03-1G4

Concentration

0.3 mg/ml

Product Summary for BAF53A Antibody (S03-1G4)

Immunogen

A synthetic peptide of human BAF53A (Uniprot # O96019)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BAF53A Antibody (S03-1G4)

Western Blot: BAF53A Antibody (S03-1G4) [NBP3-19983]

Western Blot: BAF53A Antibody (S03-1G4) [NBP3-19983]

Western Blot: BAF53A Antibody (S03-1G4) [NBP3-19983] - Western blot detection of BAF53A in Hela (LAne 1), MCF-7 (Lane 2), HepG2 (Lane 3), U2OS (Lane 4) using NBP3-19983 (1:1000 diluted)

Applications for BAF53A Antibody (S03-1G4)

Application
Recommended Usage

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAF53A

BAF53A encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Alternate Names

actin-like 6A, actin-like protein 6A, actin-related protein 4,53 kDa BRG1-associated factor A, Actin-related protein Baf53a, Actl6, Arp4, ArpNbeta, BAF complex 53 kDa subunit, Baf53a, BAF53AACTL6, BAF53MGC5382, BRG1-associated factor 53A, hArpN beta, INO80 complex subunit K, INO80KARPN-BETA

Gene Symbol

ACTL6A

Additional BAF53A Products

Product Documents for BAF53A Antibody (S03-1G4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BAF53A Antibody (S03-1G4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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