BAFF/BLyS/TNFSF13B Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76843

Novus Biologicals, part of Bio-Techne
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NBP1-76843
NBP1-76843-0.025mg
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Key Product Details

Validated by

Tagged Protein Expression

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all BAFF/BLyS/TNFSF13B Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human BAFF/BLyS/TNFSF13B. The immunogen is located within the last 50 amino acids of BAFF/BLyS/TNFSF13B. Amino Acid Sequence: EEGDELQLAIPRENAQ

Specificity

Human BAFF/BLyS/TNFSF13B has 3 isoforms, including isoform 1 (285aa, 31kD), isoform 2 (266aa, 29kD) and isoform 3 (164aa, 17.6kD). Mouse BAFF/BLyS/TNFSF13B has 2 isoforms, including isoform 1 (309aa, 34kD) and isoform 2 (290aa, 32kD). Rat BAFF/BLyS/TNFSF13B has only one isoform (274aa, 30kD). NBP1-76843 can detect two longer human isoforms, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BAFF/BLyS/TNFSF13B Antibody - BSA Free

Western Blot: BAFF/BLyS/TNFSF13B AntibodyBSA Free [NBP1-76843]

Western Blot: BAFF/BLyS/TNFSF13B AntibodyBSA Free [NBP1-76843]

Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.
Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843]

Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843]

Immunocytochemistry/Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Analysis of BAFF in human spleen tissue with BAFF antibody at 20 ug/ml.
Western Blot: BAFF/BLyS/TNFSF13B AntibodyBSA Free [NBP1-76843]

Western Blot: BAFF/BLyS/TNFSF13B AntibodyBSA Free [NBP1-76843]

Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Analysis of human HL60 cell lysate (H) and mouse spleen tissue lysate (M) with TNFSF13B antibody. Loading: 15 ug of lysates per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate.
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Applications for BAFF/BLyS/TNFSF13B Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1-20 ug/mL

Western Blot

0.25-1 ug/mL

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAFF/BLyS/TNFSF13B

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-kappaB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF and its receptors are essential for B cell development, survival, and humoral immune responses. BAFF is involved in the development of autoimmune diseases including systemic lupus erythaematosus and rheumatoid arthritis.

Long Name

B cell Activating Factor

Alternate Names

BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4

Gene Symbol

TNFSF13B

UniProt

NP_006564 (Human)

Additional BAFF/BLyS/TNFSF13B Products

Product Documents for BAFF/BLyS/TNFSF13B Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BAFF/BLyS/TNFSF13B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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