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BAG3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86429

Novus Biologicals, part of Bio-Techne
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NBP1-86429
NBP1-86429-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DCSSSSSSASLPSSGRSSLGSHQLPRGYISIPVIHEQNVTRPAAQPSFHQAQKTHYPAQQGEYQTHQPVYHKIQGDDWEPRPLRAASPFRSSVQGASSREGSPARSSTPLHSPSPIRVHTVVDRPQQPMTHRET

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAG3 Antibody

Western Blot: BAG3 Antibody [NBP1-86429]

Western Blot: BAG3 Antibody [NBP1-86429]

Western Blot: BAG3 Antibody [NBP1-86429] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Skeletal muscle tissue
Immunocytochemistry/ Immunofluorescence: BAG3 Antibody [NBP1-86429]

Immunocytochemistry/ Immunofluorescence: BAG3 Antibody [NBP1-86429]

Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP1-86429] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429]

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429]

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.

Applications for BAG3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAG3

Alternate Names

BAG family molecular chaperone regulator 3, BAG-3, BAG-family molecular chaperone regulator-3, Bcl-2-associated athanogene 3, BCL2-associated athanogene 3, BCL2-binding athanogene 3, Bcl-2-binding protein Bis, BIS, CAIR-1, Docking protein CAIR-1, MGC104307

Gene Symbol

BAG3

Additional BAG3 Products

Product Documents for BAG3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BAG3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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