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BAG3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86442

Novus Biologicals, part of Bio-Techne
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NBP1-86442
NBP1-86442-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAG3 Antibody

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442]

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442]

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human cerebellum, prostate, skeletal muscle and skin using Anti-BAG3 antibody NBP1-86442 (A) shows similar protein distribution across tissues to independent antibody NBP1-86429 (B).
Immunocytochemistry/ Immunofluorescence: BAG3 Antibody [NBP1-86442]

Immunocytochemistry/ Immunofluorescence: BAG3 Antibody [NBP1-86442]

Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP1-86442] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442]

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442]

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.

Applications for BAG3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
IHC reported in scientific literature (PMID: 30081850). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAG3

Alternate Names

BAG family molecular chaperone regulator 3, BAG-3, BAG-family molecular chaperone regulator-3, Bcl-2-associated athanogene 3, BCL2-associated athanogene 3, BCL2-binding athanogene 3, Bcl-2-binding protein Bis, BIS, CAIR-1, Docking protein CAIR-1, MGC104307

Gene Symbol

BAG3

Additional BAG3 Products

Product Documents for BAG3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BAG3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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