BAG3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86442
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BAG3 Antibody
Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442]
Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human cerebellum, prostate, skeletal muscle and skin using Anti-BAG3 antibody NBP1-86442 (A) shows similar protein distribution across tissues to independent antibody NBP1-86429 (B).Immunocytochemistry/ Immunofluorescence: BAG3 Antibody [NBP1-86442]
Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP1-86442] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442]
Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.Applications for BAG3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
IHC reported in scientific literature (PMID: 30081850). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BAG3
Alternate Names
BAG family molecular chaperone regulator 3, BAG-3, BAG-family molecular chaperone regulator-3, Bcl-2-associated athanogene 3, BCL2-associated athanogene 3, BCL2-binding athanogene 3, Bcl-2-binding protein Bis, BIS, CAIR-1, Docking protein CAIR-1, MGC104307
Gene Symbol
BAG3
Additional BAG3 Products
Product Documents for BAG3 Antibody
Product Specific Notices for BAG3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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