BAI1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-81586

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

CyTof, IF/IHC, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all BAI1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a C-terminal portion of the human BAI1 protein (within residues 1500-1584). [Swiss-Prot# O14514]

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAI1 Antibody

Western Blot: BAI1 Antibody [NB110-81586]

Western Blot: BAI1 Antibody [NB110-81586]

Western Blot: BAI1 Antibody [NB110-81586] - analysis of BAI1 in mouse brain tissue using anti-BAI1 antibody. KO mice was used as negative controls (right lane). Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: BAI1 Antibody [NB110-81586]

Immunocytochemistry/ Immunofluorescence: BAI1 Antibody [NB110-81586]

Immunocytochemistry/Immunofluorescence: BAI1 Antibody [NB110-81586] - BAI1 antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: BAI1 Antibody [NB110-81586]

Immunohistochemistry-Paraffin: BAI1 Antibody [NB110-81586]

Immunohistochemistry-Paraffin: BAI1 Antibody [NB110-81586] - A. Staining of mouse brain using NB110-81586. B. Blocking of staining using control peptide, NB110-81586PEP.

Applications for BAI1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000-1:3000

Immunohistochemistry-Paraffin

1:1000-1:3000

Western Blot

reported in scientific literature

Reviewed Applications

Read 2 reviews rated 4.5 using NB110-81586 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BAI1

BAI1 (brain-specific angiogenesis inhibitor 1) belongs to GPCR2 family or so-called adhesion-type family of 7-transmembrane receptors, and an extracellular fragment of this receptor was originally discovered to inhibit neovascularization in brain tumor model. BAI1 is a multi-pass membrane protein localized in cell membranes and is specifically expressed in brain with reduced or no expression in certain glioblastoma cell lines/cancer tissues and astrocytes. BAI1 is expressed on macrophages also, where it acts as a receptor for clearance of apoptotic cells via binding to surface phosphatidylserine of apoptotic cells through its type I TSRs followed by triggering their engulfment via an ELMO1/DOCK180/Rac1-mediated signaling pathway. BAI1 interacts with several proteins including MAGI1, MAGI3, BAIAP2, PHYHIP, ELMO1 and DOCK1. BAI1 plays a unique function in binding and internalization of Gram-negative bacteria through recognition of bacterial LPS via BAI1-TSRs. It has been proposed as a potent inhibitor of brain angiogenesis, a mediator of p53 signal in suppression of glioblastoma, and functions in cell adhesion as well as signal transduction in brain. BAI1 also regulates phagocytosis, synaptogenesis, inhibition of tumor growth and angiogenesis via processing of its extracellular domain into secreted vasculostatins, and it is emerging as a therapeutics target for diseases such as cancer, stroke, and schizophrenia.

Long Name

Brain-specific Angiogenesis Inhibitor 1

Alternate Names

brain-specific angiogenesis inhibitor 1, FLJ41988, GDAIF

Gene Symbol

ADGRB1

Additional BAI1 Products

Product Documents for BAI1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BAI1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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