BANF1 Antibody (3F10-4G12)

Novus Biologicals, part of Bio-Techne | Catalog # H00008815-M01

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F10-4G12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BANF1 Antibodies »

Product Specifications

Immunogen

BANF1 (AAH05942, 1 a.a. ~ 89 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Specificity

BANF1 - barrier to autointegration factor 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for BANF1 Antibody (3F10-4G12)

Western Blot: BANF1 Antibody (3F10-4G12) [H00008815-M01]

Western Blot: BANF1 Antibody (3F10-4G12) [H00008815-M01] - BANF1 monoclonal antibody (M01), clone 3F10-4G12 Analysis of BANF1 expression in Jurkat.

Immunohistochemistry-Paraffin: BANF1 Antibody (3F10-4G12) [H00008815-M01]

Immunohistochemistry-Paraffin: BANF1 Antibody (3F10-4G12) [H00008815-M01] - Analysis of monoclonal antibody to BANF1 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml.

Sandwich ELISA: BANF1 Antibody (3F10-4G12) [H00008815-M01] - Detection limit for recombinant GST tagged BANF1 is approximately 1ng/ml as a capture antibody.

Applications for BANF1 Antibody (3F10-4G12)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BANF1

The protein encoded by this gene was identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The endogenous function of the protein is unknown. The protein forms a homodimer which localizes to the nucleus and is specifically associated with chromosomes during mitosis. This protein binds to DNA in a non-specific manner and studies in rodents suggest that it also binds to lamina-associated polypeptide 2, a component of the nuclear lamina.

Long Name

Barrier-to-autointegration factor

Alternate Names

BCRG1

Entrez Gene IDs

8815 (Human)

Gene Symbol

BANF1

OMIM

603811 (Human)

UniProt

AAH05942 (Human)

Additional BANF1 Products

Product Documents for BANF1 Antibody (3F10-4G12)

Product Datasheets

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COA

SDS

Product Specific Notices for BANF1 Antibody (3F10-4G12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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