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BANF1 Antibody (6J8O2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16263

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16263-100ul
NBP3-16263-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6J8O2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-89 of human BANF1 (O75531). MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BANF1 Antibody (6J8O2)

Western Blot: BANF1 Antibody (6J8O2) [NBP3-16263]

Western Blot: BANF1 Antibody (6J8O2) [NBP3-16263]

Western Blot: BANF1 Antibody (6J8O2) [NBP3-16263] - Western blot analysis of extracts of various cell lines, using BANF1 Rabbit mAb (NBP3-16263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: BANF1 Antibody (6J8O2) [NBP3-16263]

Immunocytochemistry/ Immunofluorescence: BANF1 Antibody (6J8O2) [NBP3-16263]

Immunocytochemistry/Immunofluorescence: BANF1 Antibody (6J8O2) [NBP3-16263] - Immunofluorescence analysis of HeLa cells using BANF1 Rabbit mAb (NBP3-16263) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: BANF1 Antibody (6J8O2) [NBP3-16263]

Immunohistochemistry-Paraffin: BANF1 Antibody (6J8O2) [NBP3-16263]

Immunohistochemistry-Paraffin: BANF1 Antibody (6J8O2) [NBP3-16263] - Immunohistochemistry of paraffin-embedded human esophageal using BANF1 Rabbit mAb (NBP3-16263) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for BANF1 Antibody (6J8O2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BANF1

BANF1 is encoded by this gene was identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The endogenous function of the protein is unknown. The protein forms a homodimer which localizes to the nucleus and is specifically associated with chromosomes during mitosis. This protein binds to DNA in a non-specific manner and studies in rodents suggest that it also binds to lamina-associated polypeptide 2, a component of the nuclear lamina.

Long Name

Barrier-to-autointegration factor

Alternate Names

BCRG1

Gene Symbol

BANF1

Additional BANF1 Products

Product Documents for BANF1 Antibody (6J8O2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BANF1 Antibody (6J8O2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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