Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster

Cited:

Hamster

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all BANF1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 19 amino acids near the amino terminus of human BANF1. The immunogen is located within the first 50 amino acids of BANF1. Amino Acid Sequence: TTSQKHRDFVAEPMGEKPV

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 27548707).

Specificity

Human BANF1 has one isoform (89aa, 10kD). Mouse BANF1 has one isoform (89aa, 10kD) and Rat BANF1 also has one isoform (89aa, 10kD). NBP1-76751 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BANF1 Antibody - BSA Free

Western Blot: BANF1 AntibodyBSA Free [NBP1-76751]

Western Blot: BANF1 AntibodyBSA Free [NBP1-76751]

Western Blot: BANF1 Antibody [NBP1-76751] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1, NBP1-76751 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: BANF1 AntibodyBSA Free [NBP1-76751]

Western Blot: BANF1 AntibodyBSA Free [NBP1-76751]

Western Blot: BANF1 Antibody [NBP1-76751] - Analysis in Hela cell lysate with antibody at 1 ug/mL.
Western Blot: BANF1 AntibodyBSA Free [NBP1-76751]

Western Blot: BANF1 AntibodyBSA Free [NBP1-76751]

Western Blot: BANF1 Antibody [NBP1-76751] - Independent Antibody Validation via Protein Expression in Human Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1 [NBP1-76750] (2 ug/ml), BANF1 [NBP1-76751] (2 ug/ml) and beta-actin (1.5 ug/ml). 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Applications for BANF1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunofluorescence

20 ug/mL

Western Blot

1-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BANF1

Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BAF also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BAF will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BAF is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA. Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE.

Long Name

Barrier-to-autointegration factor

Alternate Names

BCRG1

Gene Symbol

BANF1

UniProt

AAH05942 (Human)

Additional BANF1 Products

Product Documents for BANF1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BANF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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