BAP28 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12652

Novus Biologicals, part of Bio-Techne
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NBP3-12652-100ul
NBP3-12652-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BAP28 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human BAP28 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for BAP28 Antibody

Western Blot: BAP28 Antibody [NBP3-12652]

Western Blot: BAP28 Antibody [NBP3-12652]

Western Blot: BAP28 Antibody [NBP3-12652] - Lane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:242 kDaObserved band size:242 kDa
Immunocytochemistry/ Immunofluorescence: BAP28 Antibody [NBP3-12652]

Immunocytochemistry/ Immunofluorescence: BAP28 Antibody [NBP3-12652]

Immunocytochemistry/Immunofluorescence: BAP28 Antibody [NBP3-12652] - Staining of HEATR1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human HEATR1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus and Cytoplasm.
Immunohistochemistry-Paraffin: BAP28 Antibody [NBP3-12652]

Immunohistochemistry-Paraffin: BAP28 Antibody [NBP3-12652]

Immunohistochemistry-Paraffin: BAP28 Antibody [NBP3-12652] - Staining of human HEATR1 in human adrenal gland with rabbit polyclonal antibody at 1:300 dilution.
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Applications for BAP28 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAP28

IRAK-4 is a member of the interleukine-1 receptor-associated kinase (IRAK) family. The IRAK family is implicated in the Toll-like receptor (TLR) and Il-1R signaling pathway (1). Upon phosphorylation, IRAK-4 interacts with IRAK-1, MYD88 and TRAF6 to form a complex, and activate NF-kB and MAPK pathways (2-3). IRAK-4 phosphorylates and activates IRAK-1 at thr387 and is also known to interact with IRAK-1 but not with the other IRAK members. Additionally, like IRAK-1, IRAK-4 possesses an auto and cross-phosphorylation kinase activity (4).

Alternate Names

BAP28HEAT repeat-containing protein 1, EC 4.1.1.31, EC 6.3.4.4, FLJ10359, HEAT repeat containing 1, MGC72083, Protein BAP28

Gene Symbol

HEATR1

Additional BAP28 Products

Product Documents for BAP28 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BAP28 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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