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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human BCAS4. The immunogen is located within the first 50 amino acids of BCAS4. Amino Acid Squence: DADQPEPMRSGARE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BASC4 Antibody - BSA Free

Western Blot: BASC4 AntibodyBSA Free [NBP1-77126]

Western Blot: BASC4 AntibodyBSA Free [NBP1-77126]

Western Blot: BASC4 Antibody [NBP1-77126] - 3T3 cell lysate with BCAS4 antibody at (A) 1 and (B) 2 ug/ml.
BASC4 Antibody - BSA Free

Immunohistochemistry: BASC4 Antibody - BSA Free [NBP1-77126] -

Immunohistochemistry: BASC4 Antibody - BSA Free [NBP1-77126] - Immunohistochemistry of BASC4 in human breast carcinoma with BASC4 antibody at 5 u/mL.
BASC4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BASC4 Antibody - BSA Free [NBP1-77126] -

Immunocytochemistry/ Immunofluorescence: BASC4 Antibody - BSA Free [NBP1-77126] - Immunofluorescence of BASC4 in human breast carcinoma tissue with BASC4 antibody at 20 u/mL.

Applications for BASC4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BASC4

The Breast carcinoma amplified sequence (BCAS4) gene is localized to 20q13, a region that is often amplified in breast cancers. It was isolated by the cloning of a highly overexpressed 1.3 kb transcript from the fusion of the last two exons of the BCAS3 and BCAS4 genes. This BCAS3-BCAS4 fusion transcript is only found in the MCF7 cell line, but the BCAS4 gene is also overexpressed in nine of thirteen breast cancer cell lines. The role of BCAS4 is unknown but its mRNA is highly expressed in thymus, spleen, kidney, brain, and placenta, suggesting BCAS4 plays other roles in multiple tissues.

Alternate Names

BCAS4/BCAS3 fusion, breast carcinoma amplified sequence 4, breast carcinoma amplified sequence 4/3 fusion protein, breast carcinoma-amplified sequence 4, CNOL, FLJ20495

Gene Symbol

BCAS4

Additional BASC4 Products

Product Documents for BASC4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BASC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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