Bat Immunoglobulin Antibody (BT1-4F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23483

Novus Biologicals, part of Bio-Techne
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NBP2-23483
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Key Product Details

Species Reactivity

Validated:

Bat

Cited:

Bat

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BT1-4F10

Concentration

1.0 mg/ml

View all Bat Immunoglobulin Antibodies »

Product Specifications

Immunogen

Microchiropteran bat (Eptesicus fuscus) spleen cells.

Reactivity Notes

Microchiropteran bats (confirmed against big brown, little brown, Red, Silverhair, Hoary bats) and porcine. Does not react with Megachiropteran bats (confirmed against P. poliocephalus, P. giganteus, P. rodricensis, R. aegyptiacus, P. vampyrus, E. helvum, P.pumilus, P. hypomelanus), human, mouse, rat, hamster, canine, feline, guinea pig, and bovine.

Specificity

Immunoglobulin L (Light) chain.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Bat Immunoglobulin Antibody (BT1-4F10)

Western Blot: Bat Immunoglobulin Antibody (BT1-4F10) [NBP2-23483]

Western Blot: Bat Immunoglobulin Antibody (BT1-4F10) [NBP2-23483]

Western Blot: Bat Immunoglobulin Antibody (BT1-4F10) [NBP2-23483] - Analysis of whole serum as detected by NBP2-23483. Left side shows the non-reduced lane, mainly IgG because all the chains are attached to each other. The right side is the reduced lane, where the different chains are separated and the one directly bound by the mAb (Light chain) is visualized.

Applications for Bat Immunoglobulin Antibody (BT1-4F10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

0.5 ug/ml-1.0 ug/ml
Application Notes
This Bat Immunoglobulin (BT1-4F10) antibody is useful for Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Flow Cytometry. This antibody is useful for detecting rabies in bats and identifies all immunoglobulin classes: IgG, IgD, IgE, IgA and IgM.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bat Immunoglobulin

The anti-bat (BT1-4F10) monoclonal antibody is a unique antibody that is able to distinguish between rabies infected versus non-infected bats in an indirect immunofluorescence assay commonly used to detect rabies but never before in bats. One of the problems with studying bat immunology is that no serologic assay exists to probe bat responses to various pathogens such as rabies, severe acute respiratory syndrome (SARS) and Geomyces destructans, the etiologic agent of white nose syndrome, a condition associated with an unprecedented bat mortality event in the United States. This antibody is helping fill that gap. Emerging pathogens, many of them viruses, continue to surprise researchers by an underappreciated source, bats. Although bats are among the most abundant, diverse and geographically dispersed orders of terrestrial mammals, typically little has been studied about the bat as a host carrying microorganisms and transmitting pathogens to other species. In fact, bats are increasingly proving to be rich reservoirs of emerging viruses and re-emerging viruses as people disrupt by encroaching natural areas exposing not only ourselves, but our domesticated animals to viruses that earlier lacked opportunities to jump species.

Additional Bat Immunoglobulin Products

Product Documents for Bat Immunoglobulin Antibody (BT1-4F10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bat Immunoglobulin Antibody (BT1-4F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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