Bax Antibody (2D2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33092
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Monkey, Mouse (Negative), Rat (Negative)
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2D2
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein. (Uniprot: Q07812)
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Cytoplasmic
Specificity
Recognizes a protein of 21kDa, identified as the Bax protein. This monoclonal antibody is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
Marker
Apoptosis Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29468).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Bax Antibody (2D2) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Bax Antibody (2D2) - Azide and BSA Free [NBP2-33092]
Immunocytochemistry/Immunofluorescence: Bax Antibody (2D2) - Azide and BSA Free [NBP2-33092] - Confocal Immunofluorescence of Human Jurkat cells. A. Bax Antibody (2D2) B. Goat anti Mouse CF488. labeled with CF488 (Green); Red Dot is used to label the nuclei.Immunohistochemistry-Paraffin: Bax Antibody (2D2) - Azide and BSA Free [NBP2-33092]
Immunohistochemistry-Paraffin: Bax Antibody (2D2) - Azide and BSA Free [NBP2-33092] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with Bax 2D2 antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Perform antigen retrieval by boiling tissue sections in 10mM citrate buffer, pH 6.0. Incubate the 2D2 antibody forFlow Cytometry: Bax Antibody (2D2) - Azide and BSA Free [NBP2-33092]
Flow Cytometry: Bax Antibody (2D2) - Azide and BSA Free [NBP2-33092] - Flow Cytometric Analysis of human Jurkat cells using Bax Antibody (2D2) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).Applications for Bax Antibody (2D2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Bax
Long Name
Bcl Associated X Protein
Alternate Names
apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4
Gene Symbol
BAX
Additional Bax Products
Product Documents for Bax Antibody (2D2) - Azide and BSA Free
Product Specific Notices for Bax Antibody (2D2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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