BCAR3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-825

Novus Biologicals, part of Bio-Techne
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NB300-825
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Canine (100%), Human (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all BCAR3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RKLEPPPVKQAEL corresponding to C-Terminus according to NP_003558.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for BCAR3 Antibody

Western Blot: BCAR3 Antibody [NB300-825]

Western Blot: BCAR3 Antibody [NB300-825]

Western Blot: BCAR3 Antibody [NB300-825] - Analysis of BCAR3 in Mouse Kidney epithelial cells lysate (15ug protein in RIPA buffer) using this antibody at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for BCAR3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: An anonymous customer has reported an approx. 110 kDa band observed in mouse embryonic kidney epithelial lysates (calculated MW of 92.6 kDa band according to human NP_003558.1 and 92.3 kDa band according to mouse NP_038895.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BCAR3

In breast cancer, the antiestrogen tamoxifen has been prescribed for both primary treatment and the treatment of advanced metastatic disease. Although the drug induces remission in most patients with estrogen receptor-positive disease, all patients eventually develop resistance. van Agthoven et al. (1998) applied an insertional mutagenesis strategy to a breast cancer cell line. Infected cells were subjected to tamoxifen selection, and the resistant clones were screened for a common integration site linked with antiestrogen resistance. By screening a human testis cDNA library with the integration site-specific probe, they obtained a cDNA encoding BCAR3 (breast cancer antiestrogen resistance 3). The deduced 825 amino acid BCAR3 protein contains a putative Src homology 2 (SH2) domain and sequences homologous to yeast CDC48. Northern blot analysis detected abundant expression of a 3.4kb BCAR3 transcript in heart, placenta, skeletal muscle, spleen, prostate, testis, ovary, small intestine, colon, fetal kidney, and several cancer cell lines, but not in nonmalignant breast tissue; a 6kb BCAR3 transcript was also detected in skeletal muscle and heart.

Alternate Names

breast cancer estrogen resistance 3, dJ1033H22.2 (breast cancer estrogen resistance 3), KIAA0554, Novel SH2-containing protein 2, NSP2SH2D3Bbreast cancer estrogen resistance protein 3, SH2 domain-containing protein 3B

Entrez Gene IDs

8412 (Human)

Gene Symbol

BCAR3

UniProt

NP_003558. (Human)

Additional BCAR3 Products

Product Documents for BCAR3 Antibody

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Certificate of Analysis

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Product Specific Notices for BCAR3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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