Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3F5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BCAT1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

42.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BCAT1 Antibody (OTI3F5)

Western Blot: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Western Blot: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - Analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-BCAT1 monoclonal antibody. (1:200)
Immunohistochemistry-Paraffin: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Immunohistochemistry-Paraffin: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human tonsil using anti-BCAT1 mouse monoclonal antibody.
Western Blot: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Western Blot: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAT1.
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Applications for BCAT1 Antibody (OTI3F5)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BCAT1

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq].

Long Name

Branched Chain Amino Acid Transaminase 1

Alternate Names

BCAT-1, BCATC, BCT1, EC 2.6.1.42, ECA39, PNAS121, PP18

Entrez Gene IDs

586 (Human)

Gene Symbol

BCAT1

Additional BCAT1 Products

Product Documents for BCAT1 Antibody (OTI3F5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BCAT1 Antibody (OTI3F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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