BCKDHA Antibody (HL2405) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25380
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Zebrafish
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2405
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human BCKDHA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BCKDHA Antibody (HL2405) - Azide and BSA Free
Western Blot: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] -
Western Blot: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] -
Western Blot: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] -
Immunocytochemistry/Immunofluorescence: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - BCKDHA antibody [HL2405] detects BCKDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCKDHA stained by BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .Applications for BCKDHA Antibody (HL2405) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BCKDHA
Alternate Names
2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDE1A, BCKDH E1-alpha, branched chain keto acid dehydrogenase E1, alpha polypeptide, branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urinedisease), Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, EC 1.2.4.4,2-oxoisovalerate dehydrogenase (lipoamide), FLJ45695, MSU, MSUD1, OVD1A
Gene Symbol
BCKDHA
Additional BCKDHA Products
Product Documents for BCKDHA Antibody (HL2405) - Azide and BSA Free
Product Specific Notices for BCKDHA Antibody (HL2405) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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