Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI11C9

Concentration

0.8 mg/ml

View all BCKDK Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BCKDK (NP_005872) produced in HEK293T cell.

Reactivity Notes

Use in Mouse reported in scientific publication (PMID: 32561715). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), nuclear gene encoding mitochondrial protein, transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BCKDK Antibody (OTI11C9)

Western Blot: BCKDK Antibody (OTI11C9) [NBP1-47664]

Western Blot: BCKDK Antibody (OTI11C9) [NBP1-47664]

Western Blot: BCKDK Antibody (11C9) [NBP1-47664] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK.
Immunocytochemistry/Immunofluorescence: BCKDK Antibody (11C9) [NBP1-47664] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCKDK.
Immunohistochemistry-Paraffin: BCKDK Antibody (OTI11C9) [NBP1-47664]

Immunohistochemistry-Paraffin: BCKDK Antibody (OTI11C9) [NBP1-47664]

Immunohistochemistry-Paraffin: BCKDK Antibody (11C9) [NBP1-47664] - Staining of paraffin-embedded Human Ovary tissue using anti-BCKDK mouse monoclonal antibody.
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Applications for BCKDK Antibody (OTI11C9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.8 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BCKDK

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex,the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex .

Alternate Names

BCKDHKIN, branched chain alpha-ketoacid dehydrogenase kinase, branched chain ketoacid dehydrogenase kinase, EC 2.7.11, EC 2.7.11.4, mitochondrial

Entrez Gene IDs

10295 (Human)

Gene Symbol

BCKDK

Additional BCKDK Products

Product Documents for BCKDK Antibody (OTI11C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BCKDK Antibody (OTI11C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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