Bcl-10 Antibody (BL10/411) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33190

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # BL10/411

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Bcl-10 Antibodies »

Product Specifications

Immunogen

Human Bcl-10 recombinant protein (epitope aa122-168) (Uniprot: O95999)

Localization

Nuclear and cytoplasmic

Marker

MALT-Lymphoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29422).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Bcl-10 Antibody (BL10/411) - Azide and BSA Free

Western Blot: Bcl-10 Antibody (BL10/411)Azide and BSA Free [NBP2-33190]

Western Blot: Bcl-10 Antibody (BL10/411)Azide and BSA Free [NBP2-33190]

Western Blot: Bcl-10 Antibody (BL10/411) - Azide and BSA Free [NBP2-33190] - HepG2 Cell Lysate using Bcl-10 Monoclonal Antibody (BL10/411).
Immunohistochemistry-Paraffin: Bcl-10 Antibody (BL10/411) - Azide and BSA Free [NBP2-33190]

Immunohistochemistry-Paraffin: Bcl-10 Antibody (BL10/411) - Azide and BSA Free [NBP2-33190]

Immunohistochemistry-Paraffin: Bcl-10 Antibody (BL10/411) - Azide and BSA Free [NBP2-33190] - Formalin-paraffin human tonsil stained with Bcl-10 Ab (BL10/411).
Flow Cytometry: Bcl-10 Antibody (BL10/411) - Azide and BSA Free [NBP2-33190]

Flow Cytometry: Bcl-10 Antibody (BL10/411) - Azide and BSA Free [NBP2-33190]

Flow Cytometry: Bcl-10 Antibody (BL10/411) - Azide and BSA Free [NBP2-33190] - Flow Cytometric Analysis of PFA-fixed K562 cells using Bcl-10 Antibody (BL10/411) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Applications for Bcl-10 Antibody (BL10/411) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Bcl-10

Bcl-10 is an adaptor protein with a caspase recruitment domain (CARD) that promotes apoptosis and activates pro-caspase 9 and NF-kB (1-2). Bcl-10 also interacts with other CARD domain containing proteins including CARD9, 10, 11 and 14. Bcl-10 is found to form a complex with MALT1, a protein known to be translocated in MALT lymphomas. Bcl-10 and MALT1 play a critical role in antigen receptor

Long Name

B Cell Lymphoma/Leukemia 10

Alternate Names

Bcl10, c-E10, CARMEN, CIPER, CLAP, mE10

Gene Symbol

BCL10

Additional Bcl-10 Products

Product Documents for Bcl-10 Antibody (BL10/411) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bcl-10 Antibody (BL10/411) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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