Bcl-2 related protein A1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76715

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Bcl-2 related protein A1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Bfl-1. The immunogen is located within the last 50 amino acids of Bfl-1. Amino Acid Squence: ENGFVKKFEPKSG

Specificity

At least two isoforms of Bfl-1 are known to exist; this antibody will only detect the larger isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bcl-2 related protein A1 Antibody - BSA Free

Western Blot: Bcl-2 related protein A1 AntibodyBSA Free [NBP1-76715]

Western Blot: Bcl-2 related protein A1 AntibodyBSA Free [NBP1-76715]

Western Blot: Bcl-2 related protein A1 Antibody [NBP1-76715] - Analysis of Bfl-1 in (A) human kidney and (B) human lung tissue lysate with Bfl-1 antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: Bcl-2 related protein A1 Antibody - BSA Free [NBP1-76715]

Immunocytochemistry/ Immunofluorescence: Bcl-2 related protein A1 Antibody - BSA Free [NBP1-76715]

Immunocytochemistry/Immunofluorescence: Bcl-2 related protein A1 Antibody [NBP1-76715] - Mouse Kidney cells.
Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody - BSA Free [NBP1-76715]

Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody - BSA Free [NBP1-76715]

Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody [NBP1-76715] - Mouse kidney tissue with Bcl2A1 antibody at 10 ug/ml.
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Applications for Bcl-2 related protein A1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl-2 related protein A1

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist. Presumably due to post-translational modifications, Bfl-1 is often observed at higher molecular weight in SDS-PAGE than its predicted molecular weight would suggest.

Alternate Names

BCL2A1, Bcl2L5, Bfl-1, GRS

Gene Symbol

BCL2A1

UniProt

NP_004040 (Human)

Additional Bcl-2 related protein A1 Products

Product Documents for Bcl-2 related protein A1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bcl-2 related protein A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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