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Bcl-9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30472

Novus Biologicals, part of Bio-Techne
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NBP2-30472
NBP2-30472-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Bcl-9 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FTGAQSGGPQQNPGVLDGPQKKPEGPIQAMMAQSQSLGKGPGPRTDVGAPFGPQGHRDVPFSPDEMVPPSMNSQSGTIGPDHLDHMTPEQIAWLKLQQEFYEEKRRKQEQVVVQQCSLQD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bcl-9 Antibody

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Analysis in human fallopian tube and pancreas tissues. Corresponding BCL9 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Bcl-9 Antibody [NBP2-30472]

Immunocytochemistry/ Immunofluorescence: Bcl-9 Antibody [NBP2-30472]

Immunocytochemistry/Immunofluorescence: Bcl-9 Antibody [NBP2-30472] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Applications for Bcl-9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl-9

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

Long Name

B Cell Lymphoma/Leukemia 9, Nuclear Co-factor of beta-Catenin Signaling

Alternate Names

Bcl9, BCL9L, LGS

Gene Symbol

BCL9

UniProt

Additional Bcl-9 Products

Product Documents for Bcl-9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Bcl-9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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