Bcl G Antibody (6R9F3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15746

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15746-100ul
NBP3-15746-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6R9F3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Bcl G Antibodies »

Product Summary for Bcl G Antibody (6R9F3)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Bcl G (Q9BZR8). MCSTSGCDLEEIPLDDDDLNTIEFKILAYYTRHHVFKSTPALFSPKLLRTRSLSQRGLGNCSANESWTEVSWPCRNSQSSEKAINLGKKKSSWKAFFGVVEKEDSQSTPAKVSAQGQRTLEYQDSHSQQWSRCLSNVEQCLEHEAVDPKVISIANRVAEIVYSWPPPQATQAGGFKSKEIFVTEGLSFQLQGHVPVASSSKKDEEEQILAKIVELLKYSGDQLERKLKKDKALMGHFQDGLSYSVFKTIT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bcl G Antibody (6R9F3)

Western Blot: Bcl G Antibody (6R9F3) [NBP3-15746]

Western Blot: Bcl G Antibody (6R9F3) [NBP3-15746]

Western Blot: Bcl G Antibody (6R9F3) [NBP3-15746] - Western blot analysis of extracts of various cell lines, using BCL2L14/Bcl G antibody (NBP3-15746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: Bcl G Antibody (6R9F3) [NBP3-15746]

Immunohistochemistry-Paraffin: Bcl G Antibody (6R9F3) [NBP3-15746]

Immunohistochemistry-Paraffin: Bcl G Antibody (6R9F3) [NBP3-15746] - Immunohistochemistry of paraffin-embedded mouse stomach using BCL2L14/Bcl G Rabbit mAb (NBP3-15746) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: Bcl G Antibody (6R9F3) [NBP3-15746]

Western Blot: Bcl G Antibody (6R9F3) [NBP3-15746]

Western Blot: Bcl G Antibody (6R9F3) [NBP3-15746] - Western blot analysis of extracts of various cell lines, using BCL2L14/Bcl G antibody (NBP3-15746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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Applications for Bcl G Antibody (6R9F3)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl G

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.

Alternate Names

apoptosis facilitator Bcl-2-like protein 14, Apoptosis regulator Bcl-G, Bcl2-L-14, BCL2-like 14 (apoptosis facilitator), BCL-G, BCLGbcl2-L-14

Gene Symbol

BCL2L14

Additional Bcl G Products

Product Documents for Bcl G Antibody (6R9F3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bcl G Antibody (6R9F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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