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bcl-x Antibody (BCL2L1/1762)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07416

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # BCL2L1/1762

Concentration

0.2 mg/ml

Product Summary for bcl-x Antibody (BCL2L1/1762)

Immunogen

Recombinant human bcl-x protein fragment (around aa 1-135) (exact sequence is proprietary) (Uniprot: Q07817)

Localization

Cytoplasmic and cell / nuclear membrane

Specificity

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241-amino acid protein which suppresses cell death. And Bcl-XS (short), a 178-amino acid protein lacking a 63-amino acid domain which functions as a dominant inhibitor of Bcl-2.

Marker

Apoptosis Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08580)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for bcl-x Antibody (BCL2L1/1762)

Application
Recommended Usage

ELISA

1-5 ug/ml
Application Notes
ELISA: For coating use Ab at 1-5ug/ml, order Ab without BSA.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Bcl-x

Bcl-x is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Alternative splicing results in two distinct Bcl-x isoforms. Bcl-xL (long) is an anti-apoptotic protein that prevents release of Cytochromec from the mitochondrial intermembrane space into the cytosol. Bcl-xs (short) is a pro-apoptotic member that can initiate programmed cell death.

Long Name

B Cell Lymphoma/Leukemia x

Alternate Names

BCL2L1, Bclx

Gene Symbol

BCL2L1

UniProt

Additional Bcl-x Products

Product Documents for bcl-x Antibody (BCL2L1/1762)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for bcl-x Antibody (BCL2L1/1762)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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