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BCL9-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14350

Novus Biologicals, part of Bio-Techne
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NBP2-14350
NBP2-14350-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: MSMCHPGQMSLLGRTGVPPQQGMVPHGLHQGVMSPPQGLMTQQNFMLMKQRGVGGEVYSQPPHMLSPQGSLMGPPPQQNLMVSHPLRQRSVSLDSQMGYLPAP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCL9-2 Antibody

Western Blot: BCL9-2 Antibody [NBP2-14350]

Western Blot: BCL9-2 Antibody [NBP2-14350]

Western Blot: BCL9-2 Antibody [NBP2-14350] - Analysis in human cell lines A-549 and HEK293 using anti-BCL9L antibody. Corresponding BCL9L RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Immunocytochemistry/ Immunofluorescence: BCL9-2 Antibody [NBP2-14350]

Immunocytochemistry/ Immunofluorescence: BCL9-2 Antibody [NBP2-14350]

Immunocytochemistry/Immunofluorescence: BCL9-2 Antibody [NBP2-14350] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BCL9-2 Antibody [NBP2-14350]

Immunohistochemistry-Paraffin: BCL9-2 Antibody [NBP2-14350]

Immunohistochemistry-Paraffin: BCL9-2 Antibody [NBP2-14350] - Staining of human liver shows no positivity in hepatocytes as expected.

Applications for BCL9-2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl9-2

BCL9L - B-cell CLL/lymphoma 9-like

Long Name

B Cell Lymphoma/Leukemia 9-like Protein

Alternate Names

Bcl92, DLNB11

Gene Symbol

BCL9L

Additional Bcl9-2 Products

Product Documents for BCL9-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BCL9-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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